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Simulation dashboard

SARS-CoV-2 Spike glycoprotein · 500 ns production run
Backbone RMSD
1.42 Å
at current frame
Radius of gyration
18.7 Å
compactness
H-bonds
214
intra-protein
Total energy
−4.81e5
kJ/mol

RMSD convergence

Backbone deviation from the starting structure

System energy

Potential energy stabilizing over the trajectory

System composition

As prepared for production

RMSD

Root-mean-square deviation vs. the reference frame
Mean (backbone)
1.31 Å
Max
2.04 Å
Plateau from
~120 ns
Converged
Yes

RMSD over time

Backbone, all-atom, and ligand traces

RMSF

Per-residue fluctuation — flexible loops & termini stand out

Fluctuation per residue

Higher values indicate greater mobility

Radius of gyration

Overall compactness of the fold

Rg over time

A stable Rg suggests the fold is maintained

SASA

Solvent-accessible surface area

SASA over time

Total surface split into hydrophobic and hydrophilic

Hydrogen bonds

Intra-protein H-bond network over time

H-bond count

Number of donor–acceptor pairs per frame

Most occupied pairs

Fraction of frames each bond is present

Contact map

Mean residue–residue distances across the trajectory

Cα–Cα contact matrix

Brighter = closer contact (Å)
0 Å
25 Å

Energy

Potential, kinetic and total energy

Energy terms over time

Stable total energy indicates an equilibrated system

Temperature & pressure

Thermostat at 310 K · barostat at 1 bar

Temperature

Target 310 K (NPT ensemble)

Pressure

Target 1 bar

PCA / Essential dynamics

Projection of the trajectory onto its two largest motions

PC1 vs PC2

Each point is a frame, colored by time (blue → cyan)

Eigenvalue scree

Variance captured by each principal component

Free-energy landscape

−kBT ln P(PC1, PC2) — basins are stable states

Energy surface (PC1 × PC2)

Dark blue basins are low-energy conformational states
0 kcal/mol
high

Secondary structure

DSSP-style content over the trajectory

Helix / sheet / coil fraction

Stacked fraction of residues per frame
α-helix β-sheet turn / coil

Ramachandran plot

Backbone φ/ψ dihedral distribution

φ vs ψ

Clusters in α-helix and β-sheet regions